Development of reliable blood tests for the detection of gene doping after intramuscular injection of recombinant adeno-associated viral vectors

Code: 11C9RS

Specific Aims 
Aim 1: Evaluate a combined assay format that is based on real-time PCR and ITC readout with a nested PCR pre-step to improve sensitivity. 
Aim 2: Assay analytical validation for specificity, sensitivity, and ruggedness (multiple labs, multiple operators, repeats and replicates) and preliminary pre-clinical performance validation.